Import carnosine

Hi, i have a question, I want to import metabolite “carnosine” in Osprey and I don’t understand how… I know in the guide osprey there are a command fit_makeBasis but I don’t know how use it…
I work with Siemens 3T…
:slight_smile:

Hi Justine,

We’ll need a bit more information here.
Import from where or which format? Do you have a complete basis set that includes carnosine? If yes, in what format? Or do you just have a simulated carnosine signal that you want to add into an existing basis set? (Also, is it carnosine or homocarnosine?)

Thanks
Georg

Hi,
Its for import from fid-a which is in .mat format we would like to modify it with the characteristics of the machine (therefore make a simulation) then put it in osprey under .rda in order to use it commonly in osprey with the graphical interface
Thanks
Justine

Hi Justine,

Apologies for letting you wait - I’ve been traveling to the ISMRM which is happening this week!
So, we’ll still need to clarify a little what you have and what you need:

  • Do you have an existing basis set or do you want to use one that Osprey has and just add a metabolite (what echo time? which sequence? which vendor?)?
  • Are you talking about carnosine or homocarnosine? We have the spin system definitions for the latter, but not for the former, we’d have to do a bit of research.

Thanks,
Georg

Hi Georg no problem !
Here is a muscle spectrum
We are interested in the carnosine peak of C2 and C4

Hi Justine,

LCModel has very specific settings for muscle spectra, and it uses internally simulated signals instead of externally simulated basis sets. We might have to add a few edits to the way that Osprey handles this kind of data, because so far we have only really done brain spectra. Mainly this will include some tailoring of the control file that will need to be fed into LCModel. Have you consulted the LCModel manual already?

Thanks,
Georg

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Hello guys @admin @justinejetu ,

I’m a new user off Osprey and I also want to import “L-Carnosine” in osprey to study muscle. Is there any edits in Osprey as you mentioned in your previous messages or I have to go through LCModel to add it.
By the way, I would like to thank you for the course online, it help me a lot to understand how to use software !!

Thanks,
Arthur

Hi Arthur,

Thanks for your kind words. I’m not really sure - the default LCModel muscle analysis (fig-muscle) doesn’t seem to include it, can you point me to a reference where this has been done? I’m not too familiar with muscle MRS.

Thanks,
Georg

Thank you for you’re answer. The study is based on this publication : https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/nbm.4266
I would like to add the two frequencies that are shown in the publication above

Thanks,
Arthur