sLASER Water Ref Question

Hello! I have data collected with Dinesh Deelchand’s Plug-and-Play sLASER sequence. I am wondering if Osprey can handle the non-water suppressed averages collected at the beginning/end of the sequence outright or if they should be separated out and entered as a separate file (like using PRESS metabolite and water data files).

Thanks so much!
Anna

Hi @AnnaKirk,
It depends a bit on the sequence version you are using and the parameter settings. Osprey can correctly identify the water scans for a specific sequence setting, but I don’t think we have all possible options covered.

Would you be able to share your files so that I can take a look?

Best,
Helge

Happy to share! What would be the most helpful for you?

Yes - That would be great!

Attaching sequence PDF from our imaging center along with an example file. Let me know if anything else would be helpful!
R21_CBD_23JAN24.pdf (190.2 KB)

Would any other files be helpful? Thanks!!

Hi @AnnaKirk,
I have tried to read in your example file with the function io_loadspec_twix which should use Osprey to read in this format. However, no water reference was recognized. Even when I have a close look at the Matrix fids, its size is 4096 x 32 x 64 (no points x coils x no averages). Normally this vector should also contain the water reference when I understand the script correctly. Hence the question: does the file certainly contains the water reference? If so, I assume that Osprey can’t take this into account.

Best,
Heiner

I checked out the data - we hadn’t had a case of this sequence yet where it acquires only one water reference before and after the water-suppressed scan (usually it acquires either 2 or 4). I’m fixing this right now.