I am wanting to confirm how the absolute quantification is calculated when using the Tarquin Gui. I have read the discussion from Absolute quantification.
When I supply a unsuppressed water file, the resulting amplitudes of metabolites are expressed in mM. I have read the Tarquin user guide, and noted the assumptions made. However, I am still unsure what assumptions (if any) are made regarding the fractions of WM/GM/CSF within the voxel.
I do not provide any T1 weighted file in Tarquin, so I am unsure if the amplitudes expressed in mM are calculated using average values for WM/GM/CSF fractions (from previous studies) or whether Tarquin is able to get this information from the SPAR/SDAT files I provided?
Any insights would be greatly appreciated.